Mary Stuart
Queen Elizabeth I
Henry Darnley
Robert Dudley
John Knox
William Cecil
Bess of Hardwick
Earl of Bothwell
David Rizzio
Earl of Lennox
Lord Maitland
James, Earl of Moray
Thomas Andrews
Earl of Kent
Earl of Shrewsbury
Mary Seton
Bull
Robert Beale
Mary Fleming
Mary Livingston
Mary Beaton
Thomas Hepburn
John Hepburn
George Dalgleish
The Laird of Ramorny
James Haliburton
Lord Randolph
Kate Carey
Thomas Jenye
William Kirkcaldy of Grange
Hector Maclean
Commoner
Sir Richard Knightley
King James I
Snuffer Woman
Fotheringhay Gentlewoman
Sir William Douglas
Young Holyrood Servant
Dorothy Stafford
Henry Killigrew
John Tamworth
William Killigrew
Portrait Painter
Andrew Ker of Fawdonside
Knox's Secretary
Francis Yaxley
Wedding Announcer
Officer
James MacDonald
Urie Campbell
Hector MacLean
Moray's Drummer
William Taylor
Bishop
Fisherman
Attaché
Sir Walter Mildmay
Soldier
Bothwell
English Nobleman (uncredited)