Miles Morales (voice)
Gwen Stacy (voice)
Jeff Morales (voice)
Rio Morales (voice)
Peter B. Parker (voice)
Miguel O'Hara (voice)
Spot (voice)
Jessica Drew (voice)
Hobie Brown (voice)
Pavitr Prabhakar (voice)
George Stacy (voice)
LYLA (voice)
Uncle Aaron (voice)
Margo Kess: Spider-Byte (voice)
Miles G. Morales (voice)
Ben Reilly (voice)
Peter Parker (voice)
Ms. Weber (voice)
Lenny (voice)
Adriano Tumino / '67 (voice)
J. Jonah Jameson (voice)
Aaron Davis
May / Quippy Spider-Person (voice)
Doc Ock (voice)
Glory (voice)
MJ (voice)
Betty (voice)
Yuri (voice)
Ganke (voice)
Mary Jane (voice)
Maria (voice)
Lego Spider-Man (voice)
Web-Slinger (voice)
Metro Spider-Man (voice)
Spectacular Spider-Man (voice)
Malala Windsor: Spider-UK (voice)
Charlotte Webber: Sun-Spider (voice)
Insomniac Spider-Man (voice)
Additional Voices (voice)
Peni Parker (voice) (uncredited)
Mrs. Chen (uncredited)