Bruce Wayne / Batman (voice)

Harvey Dent / Two-Face (voice)

Selina Kyle / Catwoman (voice)

Commissioner James Gordon (voice)

Carmine 'The Roman' Falcone / Luigi Maroni (voice)

Julian Day / Calendar Man / Oswald Cobblepot / Penguin (voice)

Gilda Dent (voice)

Alfred Pennyworth (voice)

Pamela Isley / Poison Ivy (voice)

Jonathan Crane / Scarecrow / Thomas Wayne (voice)

Jervis Tetch / Mad Hatter (voice)

Sofia Falcone (voice)

Solomon Grundy / Vincent Falcone (voice)

Barbara Gordon / Carla Viti (voice)

Joker (voice)

Renee Montoya (voice)

Pearce (voice)

Sal Maroni (voice)

Young Bruce Wayne (voice)

Bodyguard (voice)