Otis the Cow (voice)
Daisy the Cow (voice)
Ben the Cow (voice)
Miles the Mule (voice)
Bessy the Cow (voice)
Etta the Hen (voice)
Dag the Coyote (voice)
Pip the Mouse (voice)
Freddy the Ferret (voice)
Peck the Rooster / Gopher / Pizza Twin #1(voice)
Pig the Pig (voice)
Duke the Dog (voice)
Eddy the Cow (voice)
Bud the Cow / Officer O'Hanlon (voice)
Igg the Cow (voice)
Maddy the Chick (voice)
Root the Rooster (voice)
Snotty Boy / Mr. Beady / Snotty Boy's Father (voice)
Mrs. Beady (voice)
Farmer (voice)
Skunk (voice)
Snotty Boy's Mother (voice)
Snotty Boy's Friend (voice)
Snotty Boy's Friend / Additional Barnyard Voices (voice)
Barn Boys (voice)
Chick (voice)
Singer (voice)
Additional Barnyard Voices (voice)